Igk^{em1(IGK*)Abtx}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igk^{em1(IGK*)Abtx}
• Name: immunoglobulin kappa chain complex; endonuclease-mediated mutation 1, Dan Rohrer
• MGI ID: MGI:7715231
• Synonyms: Fully-Hu KLC
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Igk^{em1(IGK*)Abtx} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  human	IGK (50802)

• Mutation details: The allele has the mouse immunoglobulin kappa chain complex (Igk) region variable (V) and joining (J) segments deleted and replaced with a human IGK V-J sequence. Specifically, CRISPR/Cas9 genome editing in 129S6/SvEvTac-derived CMTI-1 embryonic stem (ES) cells was used in two separate steps to deliver into the mouse Igk locus a ~108 kb sequence comprising human IGK V-J segments (a synthetic DNA construct containing an array of 34 IGK V segments plus all 5 IGK J segments). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 33 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)