Rr350^tm1.1Mirka
Targeted Allele Detail

Summary

• Symbol: Rr350^tm1.1Mirka
• Name: regulatory region 350; targeted mutation 1.1, Mira Kassouf
• MGI ID: MGI:7714617
• Synonyms: R2-only
• Gene: Rr350 Location: Chr11:32195540-32219344 bp Genetic Position: Chr11, Syntenic
• Alliance: Rr350^tm1.1Mirka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344222
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Mutation details: Through recombinase-mediated genomic replacement (RMGR) (a type of recombinase-mediated cassette exchange, RMCE) using ESCs containing the Rhbdf1^tm1Ajhs and Hba^tm1Paz alleles and where the Hba cluster was deleted, the endogenous sequence was replaced with a synthetic BAC where hematopoietic Hba enhancers R1 (Rr351), Rm (Rr353) (all part of super-enhancer Rr349) intact. All other endogenous sequences between the Rhbdf1 and Hba allele endpoints are present. The targeted sequence has an FRT site, 5' Hprt sequences, loxP site, neomycin resistance gene cassette and a second loxP site in an intron of Rhbdf1 and a puromycin resistance gene cassette, tk gene and lox511 site in an intron of Hbq1a. The replacement BAC has a loxP site, hygromycin resistance gene cassette, a second loxP site, 3' Hprt sequences and an FRT site in the same intron of Rhbdf1 and a lox511 site, neomycin resistance gene cassette and tk gene in the same intron of Hbq1a. After Cre-mediated sequence exchange, the modified genome contains an FRT site flanked reconstituted Hprt minigene (with a loxP site in an intron) and a lox511 site in Rhbdf1 and Hbq1a, respectively. The Hprt gene was removed through subsequent Flp-mediated recombination leaving one FRT and one lox511 site. (J:344222)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr350 Mutation: 0 strains or lines available

References

• Original: J:344222 Blayney JW, et al., Super-enhancers include classical enhancers and facilitators to fully activate gene expression. Cell. 2023 Dec 21;186(26):5826-5839.e18
• All: 1 reference(s)