Rr152^em1.1Cog
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr152^em1.1Cog
• Name: regulatory region 152; endonuclease-mediated mutation 1.1, Michel Cogne
• MGI ID: MGI:7711348
• Synonyms: c3'RR
• Gene: Rr152 Location: Chr12:113191890-113217660 bp Genetic Position: Chr12, Syntenic
• Alliance: Rr152^em1.1Cog page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346952
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: The entire 30.3 kb 3'RR Igh super-enhancer was replaced with a 2.1 kb region comprising only the four core enhancers (hs3a (Rr154), hs1.2 (Rr153), hs3b (Rr155) and hs4 (Rr156) and a loxP site flanked neomycin resistance gene cassette using sgRNAs with CRISPR/Cas9 technology. The neo cassette was removed through subsequent Cre-mediated recombination. (J:346952)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr152 Mutation: 6 strains or lines available

References

• Original: J:346952 Le Noir S, et al., Functional anatomy of the immunoglobulin heavy chain 3 super-enhancer needs not only core enhancer elements but also their unique DNA context. Nucleic Acids Res. 2017 Jun 02;45(10):5829-5837
• All: 3 reference(s)