Smurf1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smurf1^{tm1c(EUCOMM)Wtsi}
• Name: SMAD specific E3 ubiquitin protein ligase 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7703616
• Gene: Smurf1 Location: Chr5:144813305-144902657 bp, - strand Genetic Position: Chr5, 84.83 cM, cytoband G2
• Alliance: Smurf1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Smurf1 gene page

Mutation origin

• Mutant Cell Line: EPD0102_3_A08
• Germline Transmission: Earliest citation of germline transmission: J:155845
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_Bact_P cassette was inserted at position 144835681 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 144836782. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smurf1 Mutation: 57 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 1 reference(s)