Rr492^em2Andg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr492^em2Andg
• Name: regulatory region 492; endonuclease-mediated mutation 2, Guillaume Andrey
• MGI ID: MGI:7666673
• Synonyms: Sensor RA3
• Gene: Rr492 Location: Chr13:56108910-56111397 bp Genetic Position: Chr13, Syntenic
• Alliance: Rr492^em2Andg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268374
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region, Reporter)
• Mutation: Insertion
• Mutation details: A DNA construct containing 50-bp of the beta-globin gene promoter upstream of the beta-galactosidase gene lacZ was inserted next to Pitx1 distal or pituitary gland enhancer PDE or Pit using an sgRNA with CRISPR/Cas9 technology. (J:268374)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr492 Mutation: 0 strains or lines available

References

• Original: J:268374 Kragesteen BK, et al., Dynamic 3D chromatin architecture contributes to enhancer specificity and limb morphogenesis. Nat Genet. 2018 Oct;50(10):1463-1473
• All: 1 reference(s)