Tm7sf3^tm1.1Ole
Targeted Allele Detail

Summary

• Symbol: Tm7sf3^tm1.1Ole
• Name: transmembrane 7 superfamily member 3; targeted mutation 1.1, Jerrold M Olefsky
• MGI ID: MGI:7657875
• Synonyms: TM7SF3^fl
• Gene: Tm7sf3 Location: Chr6:146503774-146536103 bp, - strand Genetic Position: Chr6, 77.7 cM, cytoband G3
• Alliance: Tm7sf3^tm1.1Ole page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:349456
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 146519777 of Chromosome 6 upstream of the exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 6 at position 146521997. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:349456)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tm7sf3 Mutation: 64 strains or lines available

References

• Original: J:349456 Isaac R, et al., TM7SF3 controls TEAD1 splicing to prevent MASH-induced liver fibrosis. Cell Metab. 2024 May 7;36(5):1030-1043.e7
• All: 1 reference(s)