Tyr^em4Utr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tyr^em4Utr
• Name: tyrosinase; endonuclease-mediated mutation 4, University of Tsukuba Laboratory Animal Resource Center
• MGI ID: MGI:7639911
• Synonyms: Tyr c.308G>C
• Gene: Tyr Location: Chr7:87073979-87142637 bp, - strand Genetic Position: Chr7, 49.01 cM
• Alliance: Tyr^em4Utr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Cysteine codon 103 (TGT) in exon 1 was changed to serine (TCT) (c.308G>C:p.C103S) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. (J:317830)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tyr Mutation: 383 strains or lines available

References

• Original: J:317830 Kuno A, et al., DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes. PLoS Biol. 2022 Jan;20(1):e3001507
• All: 1 reference(s)