Nrxn1^tm1.1Amcr
Targeted Allele Detail

Summary

• Symbol: Nrxn1^tm1.1Amcr
• Name: neurexin I; targeted mutation 1.1, Ann Craig
• MGI ID: MGI:7621896
• Synonyms: Nrxn1deltaHS
• Gene: Nrxn1 Location: Chr17:90341059-91400499 bp, - strand Genetic Position: Chr17, 59.73 cM
• Alliance: Nrxn1^tm1.1Amcr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277096
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codons 316 (TCG), 322 (AGC) and 333 (TCA) in exon 22 (in reference to ENSMUST00000160844) were changed to alanine (GCG, GCC, GCA) (p.S316A, p.S322A, p.S333A) and a loxP and FRT site flanked puromycin resistance gene cassette was inserted into intron 21. The puro cassette was removed through subsequent Cre-mediated recombination. The mutations, in the region of the encoded peptide where heparan sulfate (HS) is added, prevent HS-glycosylation of the affected residues. (J:277096)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrxn1 Mutation: 102 strains or lines available

References

• Original: J:277096 Zhang P, et al., Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships. Cell. 2018 Sep 6;174(6):1450-1464.e23
• All: 1 reference(s)