Actb^{em2(tetO-CHRM3*/mCherry)Kta}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actb^{em2(tetO-CHRM3*/mCherry)Kta}
• Name: actin, beta; endonuclease-mediated mutation 2, Kohichi Tanaka
• MGI ID: MGI:7610681
• Synonyms: Actb^{em2(tetO-FLEX-hM3Dq/mCherry)Kta}
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{em2(tetO-CHRM3*/mCherry)Kta} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Actb^{em2(tetO-CHRM3*/mCherry)Kta} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Actb (MGI:87904)

• Mutation details: CRISPR-targeting inserted Escherichia coli tetO, cytomegalovirus CMV minimal promoter, rabbit Beta globin intron, bacteriophage loxP, lox2272, human CHRM3, GH polyA, Discosoma sp. mCherry, Woodchuck hepatitis virus WPRE, Streptococcus pyogenes crRNA, tracrRNA (gRNA). (J:239400)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 52 strains or lines available

References

• Original: J:239400 Aida T, et al., Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. BMC Genomics. 2016 Nov 28;17(1):979
• All: 1 reference(s)