Gsdma^em#Lty
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gsdma^em#Lty
• Name: gasdermin A; endonuclease-mediated mutation, Liang-Tung Yang
• MGI ID: MGI:7580973
• Synonyms: Gsdma1^ko
• Gene: Gsdma Location: Chr11:98555177-98568534 bp, + strand Genetic Position: Chr11, 62.34 cM
• Alliance: Gsdma^em#Lty page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using 5 sgRNA 5-CTCAACTGTGACTTTCCTGA-3 and 3 sgRNA 5'-TTTCTCAAGGGGAAAGACTG-3 was used to delete exons 2-11 in cis with the Gsdma3^tm1.1Lty allele. Four founders (F1-9, F1-12, F1-16, and F1-18) were generated. F1-9 and F1-12 contain the same 11007 bp deletion, F1-16 has a 11024 bp deletion, and F1-18 has a 10998 bp deletion. This record represents pooled data from these lines or data where the line number is not specified. (J:335519)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsdma Mutation: 25 strains or lines available

Notes

This allele was generated in cis with Gsdma3^tm1.1Lty allele.

References

• Original: J:335519 Huang LY, et al., Gasdermin A Is Required for Epidermal Cornification during Skin Barrier Regeneration and in an Atopic Dermatitis-like Model. J Invest Dermatol. 2023 Mar 24
• All: 1 reference(s)