Kcnk16^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Kcnk16^{tm1c(EUCOMM)Hmgu}
• Name: potassium channel, subfamily K, member 16; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7580634
• Synonyms: Kcnk16 fl
• Gene: Kcnk16 Location: Chr14:20311444-20319267 bp, - strand Genetic Position: Chr14, 11.49 cM, cytoband B
• Alliance: Kcnk16^{tm1c(EUCOMM)Hmgu} page
• IMPC: Kcnk16 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344958
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 20313996 of Chromosome 14 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2-3 at position 20315779. The critical exons 2-3 are thus flanked by loxP sites. A "conditional ready" (exons 2-3 floxed) allele was created by flp recombinase expression which removed the lacZ and neomycin resistance gene. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:344958)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnk16 Mutation: 10 strains or lines available

References

• Original: J:344958 Graff SM, et al., TALK-1-mediated alterations of beta-cell mitochondrial function and insulin secretion impair glucose homeostasis on a diabetogenic diet. Cell Rep. 2024 Jan 23;43(1):113673
• All: 1 reference(s)