Grb7^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Grb7^{tm1c(EUCOMM)Wtsi}
• Name: growth factor receptor bound protein 7; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7572891
• Synonyms: Grb7^fl
• Gene: Grb7 Location: Chr11:98337660-98346199 bp, + strand Genetic Position: Chr11, 61.75 cM
• Alliance: Grb7^{tm1c(EUCOMM)Wtsi} page
• IMPC: Grb7 gene page

Mutation origin

• Mutant Cell Line: EPD0543_2_C04
• Germline Transmission: Earliest citation of germline transmission: J:344139
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98341112 of Chromosome 11 upstream of the critical exons 2-7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 98343225. Exons 2-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:344139)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grb7 Mutation: 18 strains or lines available

References

• Original: J:344139 Lofgren KA, et al., Grb7 knockout mice develop normally but litters born to knockout females fail to thrive. Dev Dyn. 2023 Dec 23
• All: 1 reference(s)