Igs2^{em1(CAG-HNF1A*)Plu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em1(CAG-HNF1A*)Plu}
• Name: intergenic site 2; endonuclease-mediated mutation 1, Peng Lu
• MGI ID: MGI:7572409
• Synonyms: hHNF1a^fl, hHNF1A flox
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em1(CAG-HNF1A*)Plu} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Dominant negative, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Igs2^{em1(CAG-HNF1A*)Plu} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HNF1A (6927)

  Hnf1a

  (MGI:98504)

  Contains the dominant-negative P291fsinsC mutation

• Mutation details: CRISPR/Cas9 endonuclease-mediated genome editing inserted a cassette containing the CAG promoter, loxP-flanked stop cassette, human HNF1A coding sequence containing the dominant-negative P291fsinsC mutation, and a polyA into the locus. (J:344075)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:344075 Liu M, et al., Dominant-negative HNF1alpha mutant promotes liver steatosis and inflammation by regulating hepatic complement factor D. iScience. 2023 Oct 20;26(10):108018
• All: 1 reference(s)