Prkab1^tm2.1Grst
Targeted Allele Detail

Summary

• Symbol: Prkab1^tm2.1Grst
• Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit; targeted mutation 2.1, Gregory R Steinberg
• MGI ID: MGI:7571537
• Synonyms: S108A-KI
• Gene: Prkab1 Location: Chr5:116151654-116162449 bp, - strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Prkab1^tm2.1Grst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342744
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT site and a lox5171 site flanked neomycin resistance gene cassette were inserted into intron 1 and serine codon 108 (AGC) in exon 2 and 3 was changed to alanine (GCC) (p.S108A). A loxP site, a second FRT site, a copy of the gene from exon 2 onward with intron 2 removed (and containing the p.S108A mutation in the fused exon 2-3), and an F3 site flanked puromycin resistance gene cassette and a second loxP site were inserted downstream of the gene. Subsequent Cre-mediated recombination removed the neo cassette, the duplicated sequence and puro cassette. This allele express transcripts containing the mutation on the endogenous exon structure. The mutation prevents phosphorylation of the residue in the encoded peptide. (J:342744)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkab1 Mutation: 25 strains or lines available

References

• Original: J:342744 Desjardins EM, et al., The phosphorylation of AMPKbeta1 is critical for increasing autophagy and maintaining mitochondrial homeostasis in response to fatty acids. Proc Natl Acad Sci U S A. 2022 Nov 29;119(48):e2119824119
• All: 1 reference(s)