Prkab1^tm2Grst
Targeted Allele Detail

Summary

• Symbol: Prkab1^tm2Grst
• Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit; targeted mutation 2, Gregory R Steinberg
• MGI ID: MGI:7571534
• Gene: Prkab1 Location: Chr5:116151654-116162449 bp, - strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Prkab1^tm2Grst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342744
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT site and a lox5171 site flanked neomycin resistance gene cassette were inserted into intron 1 and serine codon 108 (AGC) in exon 2 and 3 was changed to alanine (GCC) (p.S108A). A loxP site, a second FRT site, a copy of the gene from exon 2 onward with intron 2 removed (and containing the p.S108A mutation in the fused exon 2-3), and an F3 site flanked puromycin resistance gene cassette and a second loxP site were inserted downstream of the gene. Subsequent Cre-mediated recombination would remove the neo cassette and the duplicated sequence and puro cassette and create an allele expressing transcripts containing the mutation on the endogenous exon structure. Flp-mediated recombination would remove the neo cassette, endogenous exons 2-7 and the puro cassette, creating an allele expressing transcripts containing the mutation on the fused exons 2-3. (J:342744)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkab1 Mutation: 24 strains or lines available

References

• Original: J:342744 Desjardins EM, et al., The phosphorylation of AMPKbeta1 is critical for increasing autophagy and maintaining mitochondrial homeostasis in response to fatty acids. Proc Natl Acad Sci U S A. 2022 Nov 29;119(48):e2119824119
• All: 1 reference(s)