Sdcbp^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sdcbp^{tm1d(KOMP)Wtsi}
• Name: syndecan binding protein; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7564566
• Synonyms: mda-9^-
• Gene: Sdcbp Location: Chr4:6365713-6396120 bp, + strand Genetic Position: Chr4, 2.91 cM, cytoband A1
• Alliance: Sdcbp^{tm1d(KOMP)Wtsi} page
• IMPC: Sdcbp gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240369
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_st0 cassette was inserted at position 6378514 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 6379337. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:240369)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdcbp Mutation: 29 strains or lines available

References

• Original: J:240369 Das SK, et al., Knockout of MDA-9/Syntenin (SDCBP) expression in the microenvironment dampens tumor-supporting inflammation and inhibits melanoma metastasis. Oncotarget. 2016 Jul 26;7(30):46848-46861
• All: 2 reference(s)