Dnm1l^tm1.1Tac
Targeted Allele Detail

Summary

• Symbol: Dnm1l^tm1.1Tac
• Name: dynamin 1-like; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:7564260
• Synonyms: Drp1 KI, Drp1 S600A
• Gene: Dnm1l Location: Chr16:16130094-16176823 bp, - strand Genetic Position: Chr16, 10.12 cM
• Alliance: Dnm1l^tm1.1Tac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342717
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was inserted into intron 14 (with reference to ENSMUST00000023477) and human GH gene poly(A) signal sequences and a second loxP site were inserted downstream of exon 18. Genomic sequence containing the 3 end of intron 14 and the entirety of exons 15-18 and intervening introns, where serine codon 600 (TCT) in exon 16 (in ENSMUST00000023477) was changed to alanine (GCT) (p.S600A), was inserted downstream of the second loxP site. Subsequent Cre-mediated recombination removed the wild-type exons and inserted poly(A) signal sequences. The mutation blocks phosphorylation of the affected residue in the encoded peptide. (J:342717)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnm1l Mutation: 45 strains or lines available

References

• Original: J:342717 Valera-Alberni M, et al., Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation. Cell Rep. 2021 Aug 24;36(8):109565
• All: 1 reference(s)