Dnm1l^tm1Tac
Targeted Allele Detail

Summary

• Symbol: Dnm1l^tm1Tac
• Name: dynamin 1-like; targeted mutation 1, TaconicArtemis
• MGI ID: MGI:7564257
• Synonyms: Dnm1l^{tm3143(S643A)Arte}
• Gene: Dnm1l Location: Chr16:16130094-16176823 bp, - strand Genetic Position: Chr16, 10.12 cM
• Alliance: Dnm1l^tm1Tac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342717
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was inserted into intron 14 (with reference to ENSMUST00000023477) and human GH gene poly(A) signal sequences and a second loxP site were inserted downstream of exon 18. Genomic sequence containing the 3 end of intron 14 and the entirety of exons 15-18 and intervening introns, where serine codon 600 (TCT) in exon 16 (in ENSMUST00000023477) was changed to alanine (GCT) (p.S600A), was inserted downstream of the second loxP site. This allele expresses the wild-type peptide and only after conditional Cre-mediated excision of the wild-type exons will it express the modified peptide. The mutation blocks phosphorylation of the affected residue in the encoded peptide. (J:342717)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnm1l Mutation: 44 strains or lines available

References

• Original: J:342717 Valera-Alberni M, et al., Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation. Cell Rep. 2021 Aug 24;36(8):109565
• All: 1 reference(s)