Mirc31^tm1.1Arod
Targeted Allele Detail

Summary

• Symbol: Mirc31^tm1.1Arod
• Name: microRNA cluster 31, including Mirlet7c-2 through Mirlet7b; targeted mutation 1.1, Antony Rodriguez
• MGI ID: MGI:7544867
• Synonyms: let-7b^-;let-7c2^-, let-7 KO, let-7 KO strain 3
• Gene: Mirc31 Location: Chr15:85590804-85591604 bp Genetic Position: Chr15, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236467
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A targeting vector containing a loxP flanked PGK-EM7-NeobpA cassette replaced Mirlet7c-2 and Mirlet7b which make up the Mirc31 cluster. Cre-mediated recombination removed the neo cassette. PCR sequencing confirmed the presence of the 801-bp genomic deletion of Mirlet7b and Mirlet7c-2. (J:236467)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc31 Mutation: 2 strains or lines available

References

• Original: J:236467 Rowe RG, et al., Developmental regulation of myeloerythroid progenitor function by the Lin28b-let-7-Hmga2 axis. J Exp Med. 2016 Jul 25;213(8):1497-512
• All: 3 reference(s)