Src^tm2Goka
Targeted Allele Detail

Summary

• Symbol: Src^tm2Goka
• Name: src proto-oncogene, non-receptor tyrosine kinase; targeted mutation 2, Goro Kato
• MGI ID: MGI:7524671
• Gene: Src Location: Chr2:157265828-157313758 bp, + strand Genetic Position: Chr2, 78.35 cM
• Alliance: Src^tm2Goka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279554
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: A TCC to GCG change resulting in a serine to alanine change at amino acid 75 (p.S75A) in exon 2 introduced a non-phosphorylatable mutation. The targeting vector contained 9 kb of mouse Src sequence containing the 2 bp mutation, and a 3.4 kb HSV-tk-neomycin cassette flanked by a 3.2 kb duplication containing a part of the Src sequence. (J:279554)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Src Mutation: 144 strains or lines available

References

• Original: J:279554 Kashiwagi K, et al., A Ser75-to-Asp phospho-mimicking mutation in Src accelerates ageing-related loss of retinal ganglion cells in mice. Sci Rep. 2017 Dec 1;7(1):16779
• All: 2 reference(s)