Mcub^{tm1d(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Mcub^{tm1d(KOMP)Mbp}
• Name: mitochondrial calcium uniporter dominant negative beta subunit; targeted mutation 1d, Mouse Biology Program, UC Davis
• MGI ID: MGI:7513946
• Synonyms: MCUb KO
• Gene: Mcub Location: Chr3:129708609-129763850 bp, - strand Genetic Position: Chr3, 59.56 cM, cytoband H1
• Alliance: Mcub^{tm1d(KOMP)Mbp} page
• IMPC: Mcub gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338590
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 129728822 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 129728883. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:338590)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcub Mutation: 33 strains or lines available

References

• Original: J:338590 Ghatge M, et al., Mitochondrial calcium uniporter b deletion inhibits platelet function and reduces susceptibility to arterial thrombosis. J Thromb Haemost. 2023 Aug;21(8):2163-2174
• All: 1 reference(s)