Ppm1d^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ppm1d^{tm1d(KOMP)Wtsi}
• Name: protein phosphatase 1D magnesium-dependent, delta isoform; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7490129
• Synonyms: Ppm1d^KO3
• Gene: Ppm1d Location: Chr11:85202080-85237897 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Ppm1d^{tm1d(KOMP)Wtsi} page
• IMPC: Ppm1d gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308362
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85222726 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85222727. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:308362)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppm1d Mutation: 33 strains or lines available

References

• Original: J:308362 Uyanik B, et al., Inhibition of the DNA damage response phosphatase PPM1D reprograms neutrophils to enhance anti-tumor immune responses. Nat Commun. 2021 Jun 15;12(1):3622
• All: 1 reference(s)