Thsd7a^{tm1d(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Thsd7a^{tm1d(KOMP)Mbp}
• Name: thrombospondin, type I, domain containing 7A; targeted mutation 1d, Mouse Biology Program, UC Davis
• MGI ID: MGI:7488538
• Synonyms: Thsd7a^tm1d
• Gene: Thsd7a Location: Chr6:12311607-12749252 bp, - strand Genetic Position: Chr6, 5.31 cM
• Alliance: Thsd7a^{tm1d(KOMP)Mbp} page
• IMPC: Thsd7a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336253
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 12501579 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 12501591. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:336253)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thsd7a Mutation: 110 strains or lines available

References

• Original: J:336253 Seifert L, et al., The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nat Commun. 2023 Jan 28;14(1):473
• All: 1 reference(s)