Tgm2^em1Dvs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tgm2^em1Dvs
• Name: transglutaminase 2, C polypeptide; endonuclease-mediated mutation 1, David Serreze
• MGI ID: MGI:7469606
• Synonyms: NOD.TGM2 KO
• Gene: Tgm2 Location: Chr2:157958325-157988312 bp, - strand Genetic Position: Chr2, 78.72 cM
• Alliance: Tgm2^em1Dvs page

Mutation origin

• Strain of Origin: NOD/ShiLtDvs

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 genome editing is uses two guide RNAs [5';-GTCGCCGCTAGCCTGG-30 and 50- GATTTGGAGATTCAGGC-3';] to target exons 1 and 2 of the gene. Western blot analysis confirmed the absence of protein. (J:335286)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tgm2 Mutation: 49 strains or lines available

References

• Original: J:335286 Funsten MC, et al., Microbiota-dependent proteolysis of gluten subverts diet-mediated protection against type 1 diabetes. Cell Host Microbe. 2023 Feb 8;31(2):213-227.e9
• All: 1 reference(s)