Chrna5^em1Jabl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Chrna5^em1Jabl
• Name: cholinergic receptor, nicotinic, alpha polypeptide 5; endonuclease-mediated mutation 1, Julie A Blendy
• MGI ID: MGI:7468701
• Synonyms: Chrna5^A
• Gene: Chrna5 Location: Chr9:54888164-54915063 bp, + strand Genetic Position: Chr9, 29.84 cM
• Alliance: Chrna5^em1Jabl page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: Aspartic acid codon 398 (GAT) in exon 6 was changed to asparagine (AAT) (p.D398N) using an sgRNA (targeting CCGCGCYCGATTGCATTCGCTAC) and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of human SNP rs16969968 (p.D398N) associated with nicotine and opiate dependence. (J:342737)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna5 Mutation: 38 strains or lines available

References

• Original: J:342737 Brynildsen JK, et al., A common SNP in Chrna5 enhances morphine reward in female mice. Neuropharmacology. 2022 Aug 13;218:109218
• All: 1 reference(s)