Ambn^{em2(IMPC)Ccpcz}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ambn^{em2(IMPC)Ccpcz}
• Name: ameloblastin; endonuclease-mediated mutation 2, Institute of Molecular Genetics
• MGI ID: MGI:7466499
• Synonyms: Ambn^G
• Gene: Ambn Location: Chr5:88603850-88616390 bp, + strand Genetic Position: Chr5, 43.63 cM
• Alliance: Ambn^{em2(IMPC)Ccpcz} page
• IMPC: Ambn gene page

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: TALEN technology was used to replace the key residues Tyr41, Leu44, and Phe46 of the self-assembly motif with glycine residues: p.Y41G (TAT to GGA), p.L44G (CTT to GGT), and p.F46G (TTT to GGT). These residues correspond to p.Y67G, p.L70G, and p.F72G on build GRCm39. The ssODN was designed with silent mutations within the recognition sequence of Ambn-TALEN to avoid repetitive digestions. (J:241593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ambn Mutation: 32 strains or lines available

References

• Original: J:241593 Wald T, et al., Intrinsically disordered proteins drive enamel formation via an evolutionarily conserved self-assembly motif. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):E1641-E1650
• All: 2 reference(s)