Ppp1r12a^tm1.1Nju
Targeted Allele Detail

Summary

• Symbol: Ppp1r12a^tm1.1Nju
• Name: protein phosphatase 1, regulatory subunit 12A; targeted mutation 1.1, Model Animal Research Center of Nanjing University
• MGI ID: MGI:7464858
• Synonyms: Mypt1^T694A
• Gene: Ppp1r12a Location: Chr10:107997913-108115846 bp, + strand Genetic Position: Chr10, 56.33 cM, cytoband C
• Alliance: Ppp1r12a^tm1.1Nju page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:329587
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: Threonine codon 694 (ACA) in exon 15 was changed to alanine (GCA) (p.T694A) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 14. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation prevents phosphorylation of the residue. (J:329587)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp1r12a Mutation: 64 strains or lines available

References

• Original: J:329587 Chen CP, et al., In vivo roles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction. J Physiol. 2015 Feb 1;593(3):681-700
• All: 1 reference(s)