Ppm1d^tm2.1Ble
Targeted Allele Detail

Summary

• Symbol: Ppm1d^tm2.1Ble
• Name: protein phosphatase 1D magnesium-dependent, delta isoform; targeted mutation 2.1, Benjamin Ebert
• MGI ID: MGI:7461273
• Synonyms: Ppm1d^T476-fl, Ppm1d^T476*-fl
• Gene: Ppm1d Location: Chr11:85202080-85237897 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Ppm1d^tm2.1Ble page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:343530
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J x 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 5 and a second loxP site, three copies of SV40 poly(A) signal sequences, an FRT site flanked neomycin resistance gene cassette, followed by a third loxP site, the 3' end of intron 5 and a modified copy of exon 6 were inserted into the 3' UTR of the endogenous exon 6. The modification involves changing threonine codon 476 (ACT) to a stop codon (TGA) (p.T476*) and inserting sequence TTAATTAA downstream for stop codons in the other two reading frames. The neo cassette was removed through subsequent Flp-mediated recombination. Cre recombinase-mediated excision of the floxed endogenous exon 6 enables expression of the modified exon that is analogous to the somatic modifications commonly observed in humans. (J:343530)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppm1d Mutation: 32 strains or lines available

References

• Original: J:343530 Miller PG, et al., PPM1D modulates hematopoietic cell fitness and response to DNA damage and is a therapeutic target in myeloid malignancy. Blood. 2023 Dec 14;142(24):2079-2091
• All: 1 reference(s)