Notum^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Notum^{tm1b(EUCOMM)Wtsi}
• Name: notum palmitoleoyl-protein carboxylesterase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7444927
• Synonyms: Notum^lacZ
• Gene: Notum Location: Chr11:120544614-120552001 bp, - strand Genetic Position: Chr11, 84.38 cM
• Alliance: Notum^{tm1b(EUCOMM)Wtsi} page
• IMPC: Notum gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334035
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120544415 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120548038. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:334035)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notum Mutation: 30 strains or lines available

References

• Original: J:334035 Choi RB, et al., Notum Deletion From Late-Stage Skeletal Cells Increases Cortical Bone Formation and Potentiates Skeletal Effects of Sclerostin Inhibition. J Bone Miner Res. 2021 Dec;36(12):2413-2425
• All: 1 reference(s)