Flnc^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Flnc^{tm1d(EUCOMM)Hmgu}
• Name: filamin C, gamma; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7438454
• Synonyms: Flnc^gKO
• Gene: Flnc Location: Chr6:29433255-29461882 bp, + strand Genetic Position: Chr6, 12.36 cM
• Alliance: Flnc^{tm1d(EUCOMM)Hmgu} page
• IMPC: Flnc gene page

Mutation origin

• Mutant Cell Line: HEPD0690_2_H05
• Germline Transmission: Earliest citation of germline transmission: J:333147
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 29442396 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 29444686. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333147)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Flnc Mutation: 130 strains or lines available

References

• Original: J:333147 Wu T, et al., Filamin C is Essential for mammalian myocardial integrity. PLoS Genet. 2023 Jan;19(1):e1010630
• All: 1 reference(s)