Tfpi^tm1.1Madu
Targeted Allele Detail

Summary

• Symbol: Tfpi^tm1.1Madu
• Name: tissue factor pathway inhibitor; targeted mutation 1.1, Duan Ma
• MGI ID: MGI:7437558
• Synonyms: Tfpi^fl
• Gene: Tfpi Location: Chr2:84263199-84307119 bp, - strand Genetic Position: Chr2, 49.41 cM
• Alliance: Tfpi^tm1.1Madu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288197
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A reversed flipflox vector, consisting of a gene inactivation cassette (an internal ribosome entry site (IRES)-GFP reporter in reverse orientation) flanked by mutant loxP sites and an FRT-flanked PGK-neomycin selection cassette, was inserted into intron 3. The neomycin selection cassette was removed via flp-mediated recombination. The gene-inactivation cassette is transcriptionally silent and does not affect TFPI expression. Cre-mediated recombination is necessary to permanently invert the gene inactivation cassette, express EGFP and block transcription of Tfpi. (J:288197)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tfpi Mutation: 47 strains or lines available

Notes

ES cells: SCR012 (129S6/SvEv)

References

• Original: J:288197 Wang J, et al., Endothelial cell-anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice. Mol Carcinog. 2016 May;55(5):882-96
• All: 2 reference(s)