F2rl1^em1Wmrf
Endonuclease-mediated Allele Detail

Summary

• Symbol: F2rl1^em1Wmrf
• Name: F2R like trypsin receptor 1; endonuclease-mediated mutation 1, Wolfram Ruf
• MGI ID: MGI:7434618
• Synonyms: F2rl1^em1Ruf, PAR2^K36E
• Gene: F2rl1 Location: Chr13:95648240-95661735 bp, - strand Genetic Position: Chr13, 49.53 cM
• Alliance: F2rl1^em1Wmrf page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Lysine codon 36 (AAA) in exon 2 was changed to aspartic acid (GAA) (p.K36E) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation results in the resistance of the encoded peptide to skin-expressed serine proteases while remaining sensitive to cleavage by F10. (J:333152)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F2rl1 Mutation: 58 strains or lines available

References

• Original: J:333152 Fleischer MI, et al., Protease- and cell type-specific activation of protease-activated receptor 2 in cutaneous inflammation. Thromb Haemost. 2022;20(12):2823-2836
• All: 1 reference(s)