Nodal^tm5.1Rob
Targeted Allele Detail

Summary

• Symbol: Nodal^tm5.1Rob
• Name: nodal growth differentiation factor; targeted mutation 5.1, Elizabeth J Robertson
• MGI ID: MGI:7432890
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm5.1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300510
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed hygromycin cassette was inserted in intron 1 and a third loxP site was placed in intron 3. In vitro cre recombination removed the floxed hygromycin cassette from properly targeted ES cell clones. Exons 2 and 3 were then left flanked by loxP sites. Cre-mediated recombination removed exons 2 and 3. (J:300510)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 41 strains or lines available

References

• Original: J:300510 Desgrange A, et al., Transient Nodal Signaling in Left Precursors Coordinates Opposed Asymmetries Shaping the Heart Loop. Dev Cell. 2020 Nov 23;55(4):413-431.e6
• All: 2 reference(s)