Slfn3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slfn3^{tm1c(KOMP)Wtsi}
• Name: schlafen 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7427834
• Gene: Slfn3 Location: Chr11:83082156-83105980 bp, + strand Genetic Position: Chr11, 50.3 cM, cytoband B5
• Alliance: Slfn3^{tm1c(KOMP)Wtsi} page
• IMPC: Slfn3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313661
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83103025 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 83106231. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:313661)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slfn3 Mutation: 36 strains or lines available

References

• Original: J:313661 Vomhof-DeKrey EE, et al., Vil-Cre specific Schlafen 3 knockout mice exhibit sex-specific differences in intestinal differentiation markers and Schlafen family members expression levels. PLoS One. 2021;16(10):e0259195
• All: 3 reference(s)