Actb^{tm3(Kras*)Arge}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm3(Kras*)Arge}
• Name: actin, beta; targeted mutation 3, Argiris Efstratiadis
• MGI ID: MGI:7388568
• Synonyms: KRAS*A
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm3(Kras*)Arge} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217168
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Actb^{tm3(Kras*)Arge} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kras (MGI:96680)	Contains G12D mutation.

• Mutation details: An IRES and loxP site flanked neomycin resistance gene and STOP cassette were inserted along with a constitutively active Kras cDNA with the amino acid substitution of glycine with aspartic acid at position 12 (p.G12D) into intron 1. Cre-mediated removal of the STOP cassette is required to achieve expression of the constitutively active Kras. (J:217168)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 52 strains or lines available

References

• Original: J:217168 Stellas D, et al., Therapeutic effects of an anti-Myc drug on mouse pancreatic cancer. J Natl Cancer Inst. 2014 Dec;106(12)
• All: 1 reference(s)