Pgp^em1.1Mpre
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pgp^em1.1Mpre
• Name: phosphoglycolate phosphatase; endonuclease-mediated mutation 1.1, Marc Prentki
• MGI ID: MGI:7377719
• Synonyms: G3PP^flox, G3PP^lox
• Gene: Pgp Location: Chr17:24689400-24692084 bp, + strand Genetic Position: Chr17, 12.39 cM, cytoband A3.3
• Alliance: Pgp^em1.1Mpre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330347
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology flanked exons 1 and 2 with loxP sites and inserted an FRT-flanked neomycin selection cassette after exon 2. The selection cassette was removed via flp-mediated recombination in ES cells expressing flp recombinase. (J:330347, J:330198)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pgp Mutation: 6 strains or lines available

Notes

C57BL/6 ES cells contain flp recombinase.

References

• Original: J:330347 Al-Mass A, et al., Glycerol-3-phosphate phosphatase operates a glycerol shunt in pancreatic beta-cells that controls insulin secretion and metabolic stress. Mol Metab. 2022 Mar 7;60:101471
• All: 2 reference(s)