Igs2^{em62(tetO-APOBEC1*/cas9*,CAG-rtTA)Gpt}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em62(tetO-APOBEC1*/cas9*,CAG-rtTA)Gpt}
• Name: intergenic site 2; endonuclease-mediated mutation 62, GemPharmatech Co., Ltd
• MGI ID: MGI:7300675
• Synonyms: Igs2^{em1Cin(pTRE3G-YE1-BE4max-rTTA-CAG)}
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em62(tetO-APOBEC1*/cas9*,CAG-rtTA)Gpt} page

Mutation origin

• Strain of Origin: C57BL/6JGpt

Mutation description

• Allele Type: Endonuclease-mediated (Endonuclease, Inducible, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted a tetracycline response element driving inducible expression of a recombinant APOBEC1 (W90Y, R126E) fused with a cas( containing the amino acid substitution of aspartic acid with alanine at position 10 (D10A). The insertion also contains a CAG promoter dirving ubiquitous expression of a reverse tetracycline transactivator. (J:293217)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:293217 GemPharmatech, GemPharmatech Website. 2020
• All: 1 reference(s)