Gt(ROSA)26Sor^{tm1(CAG-PANX1,-EGFP)Ravi}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-PANX1,-EGFP)Ravi}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kodi S Ravichandran
• MGI ID: MGI:6888002
• Synonyms: Panx1^Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-PANX1,-EGFP)Ravi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314437
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, No functional change, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-PANX1,-EGFP)Ravi} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PANX1 (24145)

  Panx1

  (MGI:1860055)

  CAG promoter is separated from human PANX1 cDNA and EGFP gene by floxed neo cassette, so PANX1 and EGFP only expressed after cre-mediated deletion of neo. Human PANX1 has C-terminal FLAG tag sequences.

• Mutation details: A vector containing the following elements was inserted into intron 1: the CAG promoter, a loxP site, the neomycin resistance gene, a STOP cassette, a second loxP site, a human PANX1 cDNA with C-terminal FLAG tag sequence, an IRES, the EGFP reporter gene and poly(A) signal sequence. The PANX1 and EGFP genes will only be expressed after cre-mediated deletion of the neo-STOP cassette. (J:314437)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:314437 Medina CB, et al., Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammation. Immunity. 2021 Aug 10;54(8):1715-1727.e7
• All: 1 reference(s)