Dnmt3bem1Rop
Endonuclease-mediated Allele Detail
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Symbol: |
Dnmt3bem1Rop |
Name: |
DNA methyltransferase 3B; endonuclease-mediated mutation 1, Rene Opavsky |
MGI ID: |
MGI:6833877 |
Synonyms: |
Dnmt3bCI, Dnmt3bP656V/C657D |
Gene: |
Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
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Alliance: |
Dnmt3bem1Rop page
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Allele Type: |
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Endonuclease-mediated (Not Applicable) |
Mutation: |
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Nucleotide substitutions
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Mutation details: CRISPR/Cas9 technology generated a CCATGC to GTCGAC change resulting in a proline to valine substitution at amino acid 656 (P656V) and a cysteine to aspartate substitution at position 657 (C657D) in exon 19. These substitutions are predicted to disable the first two steps (nucleophilic addition and methyl transfer) of the three-step process required for catalytic activity and thus result in a catalytically inactive protein.
(J:279449)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Dnmt3b Mutation: |
50 strains or lines available
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Original: |
J:279449 Nowialis P, et al., Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions. Nat Commun. 2019 Sep 26;10(1):4374 |
All: |
3 reference(s) |
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