Mirc33^tm1Anse
Targeted Allele Detail

Summary

• Symbol: Mirc33^tm1Anse
• Name: microRNA cluster 33, including Mir29a through Mir29b-1; targeted mutation 1, K Mark Ansel
• MGI ID: MGI:6827287
• Synonyms: miR-29ab1^fl
• Gene: Mirc33 Location: Chr6:31039595-31040028 bp Genetic Position: Chr6, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304317
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop.The allele has the Mirc33 locus with loxP sites flanking the critical region for its functions, such that cre mediated recombination would produce null/knock out allele. (J:304317)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc33 Mutation: 0 strains or lines available

References

• Original: J:304317 Hines MJ, et al., miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling. Cell Rep. 2020 Dec 1;33(9):108436
• All: 1 reference(s)