Nfatc2tm1.1Ciphe
Targeted Allele Detail
|
Symbol: |
Nfatc2tm1.1Ciphe |
Name: |
nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2; targeted mutation 1.1, Centre d'ImmunoPhenomique |
MGI ID: |
MGI:6810177 |
Synonyms: |
NFATC2OST, Nfatc2tm1Ciphe |
Gene: |
Nfatc2 Location: Chr2:168318330-168443577 bp, - strand Genetic Position: Chr2, 88.91 cM
|
Alliance: |
Nfatc2tm1.1Ciphe page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:282407
|
Parent Cell Line: |
JM8.F6 (ES Cell)
|
Strain of Origin: |
C57BL/6N
|
|
Allele Type: |
|
Targeted (Epitope tag) |
Mutation: |
|
Insertion
|
|
|
Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3 end of the gene coding sequence. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification.
(J:282407)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Nfatc2 Mutation: |
53 strains or lines available
|
|
Original: |
J:282407 Voisinne G, et al., Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics. Nat Immunol. 2019 Nov;20(11):1530-1541 |
All: |
1 reference(s) |
|