Gpr161^tm2.1Smuk
Targeted Allele Detail

Summary

• Symbol: Gpr161^tm2.1Smuk
• Name: G protein-coupled receptor 161; targeted mutation 2.1, Saikat Mukhopadhyay
• MGI ID: MGI:6763974
• Synonyms: Gpr161^mut1
• Gene: Gpr161 Location: Chr1:165123358-165154314 bp, + strand Genetic Position: Chr1, 72.64 cM
• Alliance: Gpr161^tm2.1Smuk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:310417
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Single point mutation
• Mutation details: The targeting construct was generated using this engineered BAC (#RP23-235-E18) to introduce VKARK>AAAAA amino acid changes of (V/I)KARK motif in the third intracellular loop of Gpr161 in exon 4 of the gene and FRT flanked neo selection cassette into the intron following it. Correctly targeted ES cells via homologous recombination were used to establish a mouse with germline transmission and crossed to Flp-O deleted to remove the neo-cassette. (J:310417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr161 Mutation: 24 strains or lines available

References

• Original: J:310417 Hwang SH, et al., Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner. Elife. 2021 Aug 4;10:e67121
• All: 1 reference(s)