Dock8^tm2.1Ysfk
Targeted Allele Detail

Summary

• Symbol: Dock8^tm2.1Ysfk
• Name: dedicator of cytokinesis 8; targeted mutation 2.1, Yoshinori Fukui
• MGI ID: MGI:6762163
• Synonyms: Dock8^lox
• Gene: Dock8 Location: Chr19:24976898-25179796 bp, + strand Genetic Position: Chr19, 19.91 cM
• Alliance: Dock8^tm2.1Ysfk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299945
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting vector consists of mouse Dock8 gene encoding exon 3 flanked by two loxP sites, the PGK-neo cassette (Neo) flanked by two flippase recognition target (FRT) sites. After homologous recombination in ES cells and generation of chimeric male founders with correctly targetted ES cells, the PGK-neo cassette was excised by crossing with the CAG-FLPe transgenic mice. Cre-mediated excision of exon 3 results in the appearance of a premature stop codon in exon 4. (J:299945)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dock8 Mutation: 102 strains or lines available

References

• Original: J:299945 Kunimura K, et al., S100A4 Protein Is Essential for the Development of Mature Microfold Cells in Peyer's Patches. Cell Rep. 2019 Nov 26;29(9):2823-2834.e7
• All: 1 reference(s)