Tg(tetO-CCND1*K112E)#Rgp
Transgene Detail

Summary

• Symbol: Tg(tetO-CCND1*K112E)#Rgp
• Name: transgene insertion, Richard G Pestell
• MGI ID: MGI:6758517
• Synonyms: Tet-CCND1^KE
• Transgene: Tg(tetO-CCND1*K112E)#Rgp Location: unknown
• Alliance: Tg(tetO-CCND1*K112E)#Rgp page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Epitope tag, Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-CCND1*K112E)#Rgp expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CCND1 (595)

  Ccnd1

  (MGI:88313)

  human cDNA with a mutation disabling kinase activity

• Mutation details: A mutated form of the human cyclin D1 cDNA, preceded by sequence for an N-terminal 3xFLAG tag and followed by SV40 poly(A) signal sequences, was placed downstream of the tetracycline responsive element. The mutation is a G-to-A mutation that changes lysine codon 112 to a glutamic acid codon (p.K112E), eliminating or severely reducing the protein's phosphorylation (kinase) activity. (J:309458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:309458 Casimiro MC, et al., Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20;6(11):8525-38
• All: 2 reference(s)