Rr27^tm3.1Pes
Targeted Allele Detail

Summary

• Symbol: Rr27^tm3.1Pes
• Name: regulatory region 27; targeted mutation 3.1, Piroska E Szabo
• MGI ID: MGI:6753378
• Synonyms: (mChbetaGI)2, Rr27^tm4Pes
• Gene: Rr27 Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm3.1Pes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308568
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The ICR (2.4 kb BglII fragment with chromatin insulation, promoter silencing and imprinting (differential methylation) function) between the Igf2 and H19 genes was replaced with a loxP site flanked neomycin resistance gene cassette and a 2.4 kb sequence containing two copies of a mutated version of the chicken beta globin chromatin insulator ((mChbetaGI)2). The mutations deleted or disabled FP1 (VEZF1 binding site), FP3 (VEZF1), FP4 (USF binding site), and FP5 (VEZF1). The neo cassette was removed through subsequent cre-mediated recombination. (J:308568)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 6 strains or lines available

References

• Original: J:308568 Lee DH, et al., Complete biallelic insulation at the H19/Igf2 imprinting control region position results in fetal growth retardation and perinatal lethality. PLoS One. 2010 Sep 8;5(9):e12630
• All: 2 reference(s)