Zeb1^tm1Rkl
Targeted Allele Detail

Summary

• Symbol: Zeb1^tm1Rkl
• Name: zinc finger E-box binding homeobox 1; targeted mutation 1, Raghu Kalluri
• MGI ID: MGI:6719384
• Gene: Zeb1 Location: Chr18:5591860-5775467 bp, + strand Genetic Position: Chr18, 4.42 cM
• Alliance: Zeb1^tm1Rkl page
• IMPC: Zeb1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306570
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 5765734 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 5768266. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:306570)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zeb1 Mutation: 65 strains or lines available

References

• Original: J:306570 Carstens JL, et al., Stabilized epithelial phenotype of cancer cells in primary tumors leads to increased colonization of liver metastasis in pancreatic cancer. Cell Rep. 2021 Apr 13;35(2):108990
• All: 3 reference(s)