Dhcr24^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dhcr24^{tm1c(EUCOMM)Wtsi}
• Name: 24-dehydrocholesterol reductase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6715348
• Synonyms: Dhcr24^flx
• Gene: Dhcr24 Location: Chr4:106418279-106446310 bp, + strand Genetic Position: Chr4, 49.67 cM, cytoband C7
• Alliance: Dhcr24^{tm1c(EUCOMM)Wtsi} page
• IMPC: Dhcr24 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304449
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_st0 cassette was inserted at position 106428382 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 106429001. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. (J:304449)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dhcr24 Mutation: 33 strains or lines available

References

• Original: J:304449 Kanuri B, et al., Generation and validation of a conditional knockout mouse model for desmosterolosis. J Lipid Res. 2021 Jan 30;62:100028
• All: 2 reference(s)