Gabra5^tm1.1Krmr
Targeted Allele Detail

Summary

• Symbol: Gabra5^tm1.1Krmr
• Name: gamma-aminobutyric acid type A receptor subunit alpha 5; targeted mutation 1.1, Richard Kramer
• MGI ID: MGI:6693435
• Synonyms: alpha5-E125C knock-in
• Gene: Gabra5 Location: Chr7:57057420-57159807 bp, - strand Genetic Position: Chr7, 33.52 cM
• Alliance: Gabra5^tm1.1Krmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300549
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Single point mutation
• Mutation details: The allele was designed to introduce a glutamic acid-to-cysteine mutation at codon 125 (E152C counting from the start codon; or E125C in the mature peptide sequence) into exon 5 of the gene. The targeting vector also includes a loxP-flanked neomycin selection cassette upstream of exon 5, as well as a C-to-T silent mutation in the exon 5 DNA sequence (to create a HindIII site upstream of the mutation for genotyping). Cre-mediated recombination removed the floxed neo cassette. (J:300549)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabra5 Mutation: 32 strains or lines available

References

• Original: J:300549 Davenport CM, et al., Relocation of an Extrasynaptic GABAA Receptor to Inhibitory Synapses Freezes Excitatory Synaptic Strength and Preserves Memory. Neuron. 2021 Jan 6;109(1):123-134.e4
• All: 1 reference(s)