Cblb^tm1.1Ciphe
Targeted Allele Detail

Summary

• Symbol: Cblb^tm1.1Ciphe
• Name: Cbl proto-oncogene b; targeted mutation 1.1, Centre d'ImmunoPhenomique
• MGI ID: MGI:6690622
• Synonyms: CBLB^OST, Cblb^tm1Ciphe
• Gene: Cblb Location: Chr16:51851593-52028410 bp, + strand Genetic Position: Chr16, 32.88 cM
• Alliance: Cblb^tm1.1Ciphe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239186
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, an IRES2 sequence, a Kozak sequence, a sequence coding for the monomeric cyan (teal) fluorescent reporter gene mTFP1, a loxP site, and an FRT site flanked neomycin resistance gene cassette, was inserted in-frame at the 3 end of the gene coding sequence exon 19. A second loxP site was inserted into intron 18. The neo cassette was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. (J:239186)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cblb Mutation: 59 strains or lines available

References

• Original: J:239186 Voisinne G, et al., Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. Mol Syst Biol. 2016 Jul 29;12(7):876
• All: 2 reference(s)