Igs2^{em1(EIF1AX-LOC4337827)Pwj}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em1(EIF1AX-LOC4337827)Pwj}
• Name: intergenic site 2; endonuclease-mediated mutation1, Philip Jordan
• MGI ID: MGI:6682039
• Synonyms: V1-TIR1
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em1(EIF1AX-LOC4337827)Pwj} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert the V1-TIR1 construct containing from 5' to 3' a human EIF1AX (eukaryotic translation initiation factor 1A X-linked) promoter directing expression of a rice Oryza sativa TIR1 sequence with a hemagglutinin (HA) tag followed by a loxP-flanked puromycin. (J:302997)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:302997 Pryzhkova MV, et al., Adaptation of the AID system for stem cell and transgenic mouse research. Stem Cell Res. 2020 Dec;49:102078
• All: 1 reference(s)